Journal: Molecular Medicine Reports

Article Title: Heme oxygenase 1‑overexpressing bone marrow mesenchymal stem cell‑derived exosomes suppress interleukin‑1 beta‑induced apoptosis and aging of nucleus pulposus cells

doi: 10.3892/mmr.2025.13481

Figure Lengend Snippet: Incubation and characterization of BMSCs. (A) Representative images of BMSCs with spindle-like morphology under the microscope (scale bar, 100 µm). Representative images of alizarin red S staining, oil red O staining and Alcian blue staining of BMSCs differentiated into osteoblasts, adipocytes, or chondrocytes (scale bar, 100 µm). (B) Flow cytometry analysis of BMSCs for cell surface markers CD29, CD73, CD90, CD34 and CD45. BMSCs, bone marrow mesenchymal stem cells.

Article Snippet: BMSCs were incubated with CD29 (5 μl; cat. no. E-AB-F1309D, Wuhan Elabscience Biotechnology Co., Ltd.), CD73 (5 μl; cat. no. E-AB-F1089D, Wuhan Elabscience Biotechnology Co., Ltd.), CD90 (5 μl; cat. no. E-AB-F1283D, Wuhan Elabscience Biotechnology Co., Ltd.), CD34 (5 μl; cat. no. E-AB-F1284D, Wuhan Elabscience Biotechnology Co., Ltd.) and CD45 (5 μl; cat. no. E-AB-F1136D, Wuhan Elabscience Biotechnology Co., Ltd.) antibodies for 30 min at 4°C in the dark.

Techniques: Incubation, Microscopy, Staining, Flow Cytometry

Journal: Medical Oncology (Northwood, London, England)

Article Title: Ashwagandha ( Withania somnifera ) targets liver cancer stem cells via inhibiting Hedgehog signaling pathway in hepatocellular carcinoma

doi: 10.1007/s12032-025-03215-0

Figure Lengend Snippet: Flow cytometry analysis of CD90 expression in HepG2 cells treated with SOR and/or ASH-AE for 48 h. *: Statistically significant difference from control group at p ≤ 0.05, #: Statistically significant difference from SOR group at p ≤ 0.05

Article Snippet: After 24 h, cells were treated with IC50 of SOR and/or ASH-AE in triplicates and incubated in the same conditions for 48 h. After that, cells were collected and 1 × 10 6 cells were resuspended in 100 μl PBS and then incubated with PE/Elab Fluor ® 594 Anti-Human CD90 Antibody (1:50 dilution) (Elabscience, USA) for 30 min at room temperature.

Techniques: Flow Cytometry, Expressing, Control